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KMID : 1134820230520010008
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Journal of the Korean Society of Food Science and Nutrition
2023 Volume.52 No. 1 p.8 ~ p.16
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In Vitro Anticancer Effects of Unitein and Deep Seawater Salt Minerals on HT-29 Human Colon Carcinoma Cells
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Lee Kyung-Hwa
Song Woon-Seo
Lee Yeon-Jun
Pan Yanni
Hahm Suk-Chan
Park Kun-Young
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Abstract
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This study investigated the inhibitory mechanisms of Unitein (a fermented product of soybeans, red ginseng, and dried mandarin peel) and deep seawater salt minerals (DSM) on the proliferation of HT-29 cells (a human colon carcinoma cell line). An MTT assay was used to examine the effects of Unitein (Uni), unfermented Unitein (UU), and DSM on cell growth, and the mRNA expressions of genes related to the cell cycle, apoptosis, and inflammation were measured. Uni inhibited cell growth almost twice as much as UU at 1 mg/mL. DSM inhibited cell growth by 32.68±1.99% at 0.2 mg/mL, and mixtures of Uni (1 mg/mL)＋2.5% DSM (UL), and Uni (1 mg/mL)＋10% DSM (UH), inhibited growth significantly more than Uni (1 mg/mL). RT-qPCR results showed that Uni, UL, or UH significantly up-regulated the mRNA expressions of p53 and p21 (cell cycle arrest-related genes) and Bim, Bax, Bak, Bad, caspase 9, and caspase 3 (pro-apoptotic genes) and down-regulated the mRNA expressions of Bcl-2 (an anti-apoptotic gene), and NF-κB p65, NF-κB p50, and COX-2 (inflammation-related genes). The study shows Unitein and deep seawater salt minerals exert anti-cancer effects by regulating the cell cycle, apoptosis, and the expressions of inflammatory genes, and thus, inhibiting the proliferation of HT-29 cancer cells.
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KEYWORD
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Unitein, deep seawater salt minerals, cell cycle, apoptosis, inflammation
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